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with a variable appearance in shape or size&#46; This disease is classified into two subgroups&#44; generalized and localized&#44; in both of them insufficiency of melanocyte function can be observed&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">5</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">The main causes of vitiligo are complex and maybe stem from genetic disorders&#44; which can feature polygenic and multifactorial inheritance&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">6</span></a> The main cause is not yet clear&#59; however&#44; some evidence has shown that exchange in immunological programs contributed to this kind of action&#46;<a class="elsevierStyleCrossRef" href="#bib0265"><span class="elsevierStyleSup">7</span></a> Vitiligo is assumed to result from autoimmune reactions that gradually destroy melanocytes&#46; Despite the detection of self-reacting auto-antibodies in vitiligo patients&#44; increasing evidence supports impaired cellular immunity as the main cause of this disfiguring disease&#46;<a class="elsevierStyleCrossRef" href="#bib0270"><span class="elsevierStyleSup">8</span></a> Cytokines have a pivotal role as mediators of cellular and humoral immune reactions&#46; The imbalance between pro- and anti-inflammatory cytokines favoring the dominance of a Th1&#47;Th17 response rather than a Th2&#47;Treg response has been proposed as a possible underlying mechanism of vitiligo&#46; However&#44; there is considerable evidence in support of the role of CD8 type 1 T cells in the destruction of melanocytes&#46;<a class="elsevierStyleCrossRefs" href="#bib0275"><span class="elsevierStyleSup">9&#44;10</span></a> Increased expression of pro-inflammatory cytokines in vitiligo patients &#8211; including IL-1&#44; IL-6&#44; and TNF-&#945; has been shown in several studies&#46;<a class="elsevierStyleCrossRefs" href="#bib0285"><span class="elsevierStyleSup">11&#8211;13</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">IL-27&#44; a rather new member of the IL-6&#47;IL-12 family&#44; is mainly released by activated antigen-presenting cells&#44; including dendritic cells &#40;DCs&#41;&#44; monocytes&#44; and macrophages&#46;<a class="elsevierStyleCrossRefs" href="#bib0300"><span class="elsevierStyleSup">14&#44;15</span></a> It is composed of two subunits&#44; EBV-induced 3 &#40;EBI3&#41;&#44; an IL-12 p40 homolog&#44; and p28&#44; an IL-6 p35 homolog&#46; The IL-27 receptor &#40;IL-27R&#41; is a heterodimer composed of an IL-27R specific alpha chain &#40;WSX-1&#41; and a gp130 subunit&#46;<a class="elsevierStyleCrossRef" href="#bib0310"><span class="elsevierStyleSup">16</span></a> The IL-27R is expressed by several cell types including but not limited to T&#44; B&#44; and NK cells&#44; DCs&#44; macrophages&#44; keratinocytes&#44; CD8 type1 T-cells&#44; and endothelial cells&#46;<a class="elsevierStyleCrossRefs" href="#bib0315"><span class="elsevierStyleSup">17&#8211;19</span></a> IL-27R signaling results in the activation of JAK-STAT and p38 MAPK pathways&#46;<a class="elsevierStyleCrossRefs" href="#bib0330"><span class="elsevierStyleSup">20&#44;21</span></a> IL-27 boosts the differentiation of Th1 and Tr1 cells through activation of STAT1&#47;3 while inhibiting the differentiation of regulatory T-cells and Th2 cells&#46;<a class="elsevierStyleCrossRefs" href="#bib0335"><span class="elsevierStyleSup">21&#8211;26</span></a> Besides Th1 differentiation&#44; IL-27 signaling through STAT-1 has also been demonstrated to activate NK cells&#44; which have been considered to have a role in the pathogenesis of vitiligo&#46;<a class="elsevierStyleCrossRef" href="#bib0365"><span class="elsevierStyleSup">27</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Although previous studies identified the inflammatory role of IL-27&#44; the latest evidence suggests an immunomodulatory effect of this cytokine&#46;<a class="elsevierStyleCrossRef" href="#bib0370"><span class="elsevierStyleSup">28</span></a> IL-27 induces Tr1 and inhibits Th2 and Th17 responses&#44; therefore limiting the severity of autoimmune diseases by the suppression of Th17 cells&#46;<a class="elsevierStyleCrossRefs" href="#bib0375"><span class="elsevierStyleSup">29&#8211;31</span></a> It has also immune-regulatory functions due to the up-regulation of PD-L1&#44; IDO&#44; and IL-10&#46;<a class="elsevierStyleCrossRef" href="#bib0390"><span class="elsevierStyleSup">32</span></a> Thus&#44; it has suggested that this cytokine may be considered as a possible therapeutic agent for some inflammatory or autoimmune diseases&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">Regarding the complex pro-inflammatory and anti-inflammatory nature of IL-27&#44; several studies have investigated the local or systemic concentrations of IL-27 in different diseases&#46; Alteration in the concentration of IL-27 and its correlation with autoimmune parameters have also been reported in several Th1&#47;Th17-mediated inflammatory disorders&#44; such as multiple sclerosis&#44; systemic lupus erythematosus&#44; inflammatory bowel disease&#44; and rheumatoid arthritis&#46;<a class="elsevierStyleCrossRefs" href="#bib0395"><span class="elsevierStyleSup">33&#8211;36</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">The concentrations of IL-27 in vitiligo patients have not yet been investigated&#46; The present examination intended to evaluate the serum levels of IL-27 in vitiligo patients&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Study population</span><p id="par0035" class="elsevierStylePara elsevierViewall">Seventy-nine vitiligo patients including 32 &#40;41&#37;&#41; males and 47 &#40;59&#37;&#41; females&#44; and 45 age and sex-matched healthy controls with no sign of autoimmune diseases were enrolled in the study&#46; The study population included 72 patients with generalized vitiligo and seven cases with localized vitiligo&#46; Participants in this study assented to join according to the ethics committee of the medical school of Shiraz University of Medical Sciences &#40;IR&#46;sums&#46;med&#46;rec&#46;1397&#46;400&#41;&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Sample collection</span><p id="par0040" class="elsevierStylePara elsevierViewall">From all study populations&#44; 5<span class="elsevierStyleHsp" style=""></span>mL of peripheral blood was collected&#46; After centrifugation at 3000<span class="elsevierStyleHsp" style=""></span>rpm for 10<span class="elsevierStyleHsp" style=""></span>min&#44; the sera were separated and kept at &#8722;70<span class="elsevierStyleHsp" style=""></span>&#176;C until used&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Measurement of serum IL-27</span><p id="par0045" class="elsevierStylePara elsevierViewall">Serum concentrations of IL-27 were distinguished by enzyme-linked immunosorbent assay &#40;ELISA&#41; in vitiligo patients and healthy subjects&#46; IL-27 concentrations were quantified using an ELISA kit &#40;DY2526-05&#44; R&#38;D Systems &#8211; United States&#41; manufacturers&#8217; instructions&#46; The sensitivity of tests was 12&#46;8<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#46; Briefly&#44; 100<span class="elsevierStyleHsp" style=""></span>&#956;L of capture antibody was coated in each well of a 96-well microplate for overnight at room temperature &#40;RT&#41;&#46; After washing and blocking&#44; 100<span class="elsevierStyleHsp" style=""></span>&#956;L serum samples of patients and controls were added for 2<span class="elsevierStyleHsp" style=""></span>h&#44; at RT&#46; Plates were washed again and 100<span class="elsevierStyleHsp" style=""></span>&#956;L of the detection antibody and then 100<span class="elsevierStyleHsp" style=""></span>&#956;L of the working dilution of streptavidin horseradish peroxidase was added for 20<span class="elsevierStyleHsp" style=""></span>min&#46; Then substrate solution &#40;100<span class="elsevierStyleHsp" style=""></span>&#956;L&#41; was added for 20<span class="elsevierStyleHsp" style=""></span>min&#59; finally&#44; the reaction was stopped by adding stop solution to each well&#46; The optical density of samples was read using a microplate reader at 450<span class="elsevierStyleHsp" style=""></span>nm&#46; The levels of cytokine were extrapolated from the related standard curve&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Statistical analysis</span><p id="par0050" class="elsevierStylePara elsevierViewall">All data were analyzed by SPSS v&#46; 16 software &#40;SPSS Inc&#46; &#8211; Chicago&#44; IL&#44; United States&#41; and according to the normality test &#40;Kolmogorov-Smirnov test&#41;&#59; non-parametric &#40;Mann&#8211;Whitney <span class="elsevierStyleItalic">U</span> and Kruskal&#8211;Wallis&#41; tests were used&#59; <span class="elsevierStyleItalic">p</span>-values<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 were considered as significant&#46; Graphs were constructed using Graph Pad Prism &#40;v&#46; 6 &#8211; La Jolla&#44; CA&#44; United States&#41;&#46; All data are presented as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>standard error of the mean &#40;SEM&#41;&#44; unless otherwise specified&#46;</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Results</span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Demographic analysis</span><p id="par0055" class="elsevierStylePara elsevierViewall">An overview of the demographic and clinical features of vitiligo patients and healthy subjects participated in the study is provided in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Measurement of serum IL-27</span><p id="par0060" class="elsevierStylePara elsevierViewall">The serum concentrations of IL-27 in 79 patients with generalized &#40;72 cases&#41; or localized &#40;seven cases&#41; vitiligo were observed using the ELISA technique&#46; Forty-five age and sex-matched healthy subjects were used as the control group&#46; As presented in <a class="elsevierStyleCrossRef" href="#fig0005">Figure 1</a>&#44; the results showed that there was a significant difference in serum concentrations of IL-27 between vitiligo patients &#40;5267&#46;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>399<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#41; and healthy subjects &#40;7097&#46;7<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1502<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#41; &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;0262&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Correlations of IL-27 with demographic information and clinical manifestations</span><p id="par0065" class="elsevierStylePara elsevierViewall">The correlations between serum concentrations of IL-27 with gender&#44; the severity of the disease&#44; two types of &#40;localized&#47;generalized&#41; vitiligo&#44; segmental&#47;nonsegmental forms of the disease&#44; and response to treatment in patients with vitiligo were examined&#46; There was no significant correlation between IL-27 levels and these parameters in the present study &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia></span></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Discussion</span><p id="par0070" class="elsevierStylePara elsevierViewall">The main reason for vitiligo is ill defined&#46; Human and experimental studies have recently provided considerable evidence regarding the pattern of autoimmunity in the pathogenesis of vitiligo&#46; Both humoral and cellular immunity have shown to be involved in the etiology of the disease&#46;<a class="elsevierStyleCrossRefs" href="#bib0415"><span class="elsevierStyleSup">37&#8211;39</span></a> The accumulated evidence suggests a primary role for cell-mediated mechanisms&#44; including Th1&#47;Th17 and Tc1 cells in the pathogenesis of vitiligo&#46;<a class="elsevierStyleCrossRef" href="#bib0430"><span class="elsevierStyleSup">40</span></a> Various inflammatory cytokines &#40;IL-1&#44; IL-6&#44; TNF-&#945;&#44; IL-6&#44; and IL-17&#41; have a key role in skin depigmentation&#44; while the level of TGF-&#946; indicated reversible state&#46;<a class="elsevierStyleCrossRefs" href="#bib0260"><span class="elsevierStyleSup">6&#44;41</span></a></p><p id="par0075" class="elsevierStylePara elsevierViewall">IL-27 has been suggested to function as a pro-inflammatory as well as an anti-inflammatory cytokine&#46; Altered concentrations of IL-27 have been shown in several autoimmune and skin disorders&#46; Despite early studies identified pro-inflammatory role of IL-27&#44; latest evidence suggests that it suppresses a range of immune cell proliferation and cytokine production&#46;<a class="elsevierStyleCrossRef" href="#bib0370"><span class="elsevierStyleSup">28</span></a> These cytokine applications are related to its pro- or anti-inflammatory activity&#46; The dual role of IL-27 is related to the different tissues involved&#44; the underlying mechanism&#44; or the kind and stage of autoimmune diseases&#46;<a class="elsevierStyleCrossRef" href="#bib0375"><span class="elsevierStyleSup">29</span></a> Previous studies revealed that the blood concentration of IL-27 increased in pemphigus and psoriatic patients&#46; They showed that IL-27 levels strongly correlated with the IgG auto-antibody titers in pemphigus&#44; and also disease onset and severity of psoriasis&#46;<a class="elsevierStyleCrossRefs" href="#bib0440"><span class="elsevierStyleSup">42&#44;43</span></a> The expression of IL-27 in lesional eczematous skin has been reported too&#46;<a class="elsevierStyleCrossRef" href="#bib0445"><span class="elsevierStyleSup">43</span></a> This evidence supports a pro-inflammatory function of IL-27&#46; In spite of skin disorders in which IL-27 plasma concentrations have increased&#44; the serum level of this cytokine is reduced in some auto-immune diseases such as Vogt-Koyanagi-Harada syndrome &#40;VKH&#41;&#44; Beh&#231;et&#39;s disease &#40;BD&#41;&#44; and SLE&#46; Wang and his colleagues described that the expression of IL-27 p28 mRNA by peripheral blood mononuclear cells &#40;PBMCs&#41; and serum concentration of IL-27 in the sera and supernatants of cultured PBMCs were noticeably reduced in patients with active BD and VKH&#46; In addition&#44; Gaber et al&#46; have observed that the IL-27 level in SLE patients is markedly lower than in healthy controls&#46;<a class="elsevierStyleCrossRefs" href="#bib0450"><span class="elsevierStyleSup">44&#8211;46</span></a></p><p id="par0080" class="elsevierStylePara elsevierViewall">Based on those explanations and the results of several studies about the altered expression of IL-27 in autoimmunity and skin disorders&#44; it was hypothesized that the concentration of IL-27 in vitiligo patients might be altered&#46; Consistent with previous studies in SLE and BD patients&#44; the present study showed that the concentrations of IL-27 serum levels were reduced in vitiligo patients&#44; which can be justified through some probable mechanisms&#46; First&#44; IL-27 acts against Th17 development in a direct manner through modulating DCs and induces IL-10 production by na&#239;ve CD4&#43; T-cells&#46;<a class="elsevierStyleCrossRef" href="#bib0455"><span class="elsevierStyleSup">45</span></a> Th17 contributes to the pathogenesis of vitiligo by IL-17 production&#46; IL-27 inhibits the differentiation and generation of Th17 cells via IL-6 and transforming growth factor-&#946; &#40;TGF-&#946;&#41; suppression&#44; which is dependent on the intracellular signaling molecule STAT1&#46;<a class="elsevierStyleCrossRef" href="#bib0450"><span class="elsevierStyleSup">44</span></a></p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Conclusion</span><p id="par0085" class="elsevierStylePara elsevierViewall">It can be concluded that IL-27 has an immunomodulatory role in vitiligo&#46; According to the observations of the present study&#44; there was no association between IL-27 serum level and demographic information&#44; severity of the disease&#44; types of vitiligo&#44; and response to treatment in patients with vitiligo&#46; Further studies with more patients of all types of vitiligo are needed to reveal the possible alterations in the concentration of IL-27 in those patients and their correlation with disease characteristics&#44; including severity and the extension of the affected skin area&#46; In conclusion&#44; IL-27 has two roles&#59; it should be considered as a new target for the manipulation of the immune system in various immune-mediated disorders&#46;</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Financial support</span><p id="par0090" class="elsevierStylePara elsevierViewall">This study was supported by <span class="elsevierStyleGrantSponsor" id="gs1">Shiraz University of Medical Sciences</span> &#40;grant No&#46; <span class="elsevierStyleGrantNumber" refid="gs1">17684</span>&#41;&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Authors&#8217; contributions</span><p id="par0095" class="elsevierStylePara elsevierViewall">Saeed Malek Hosseini&#58; Drafting and editing of the manuscript&#46;</p><p id="par0100" class="elsevierStylePara elsevierViewall">Naser Gholijani&#58; Collection&#44; analysis&#44; and interpretation of data&#59; participation in the study design&#46;</p><p id="par0105" class="elsevierStylePara elsevierViewall">Nooshafarin Chenari&#58; Collection&#44; analysis&#44; and interpretation of data&#59; participation in the study design&#46;</p><p id="par0110" class="elsevierStylePara elsevierViewall">Nooshafarin Chenari&#58; Drafting and editing of the manuscript&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Conflicts of interest</span><p id="par0115" class="elsevierStylePara elsevierViewall">None declared&#46;</p></span></span>"
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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Background</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Vitiligo is a common skin disorder in which melanocytes are destroyed by auto-reactive immune responses&#46; The loss of melanocytes results in the appearance of depigmented areas in different parts of the body&#46; Cytokines have remarkable roles in the pathogenesis of vitiligo&#44; such as IL-1&#44; IL-6&#44; and TNF-&#945;&#59; interleukin 27 &#40;IL-27&#41; is a new member of the IL-6&#47;IL-12 family&#44; mainly released by activated antigen-presenting cells&#46; IL-27 has been suggested to function as a pro-inflammatory as well as an anti-inflammatory cytokine&#46; Altered concentrations of IL-27 have been shown in various auto-immune diseases such as multiple sclerosis&#44; rheumatoid arthritis&#44; and psoriasis&#46; No studies have been conducted to determine the expression of this cytokine in vitiligo patients&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Objective</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">The objective of this study was to determine the serum concentration of IL-27 in vitiligo patients and compare it with normal individuals&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Methods</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The serum concentration of IL-27 in 79 vitiligo patients was evaluated in comparison to 45 healthy controls using ELISA assay&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Results</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Results showed decreased concentration of IL-27 in vitiligo patients as compared with healthy subjects &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;026&#41;&#46; Furthermore&#44; no correlation between IL-27 concentrations and disease parameters such as vitiligo severity and the extension of the depigmented area was observed&#46;</p></span> <span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Study limitation</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">A larger sample size would be more recommended for this study&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Conclusion</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">The reduction in the serum levels of IL-27 in vitiligo patients compared to normal subjects suggested the possible anti-inflammatory role of this cytokine in vitiligo&#46; Thus&#44; IL-27 may be considered as a new target for the manipulation of the immune system in vitiligo patients&#46;</p></span>"
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                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">36&#46;37<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>14&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">35&#46;06<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>11&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
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                  \t\t\t\t\ttop\n
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                  \t\t\t\t  " colspan="3" align="left" valign="\n
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                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">32 &#40;41&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t\ttable-entry\n
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                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">47 &#40;59&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">27 &#40;60&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
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                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
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                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Mean &#40;pg&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">p</span>-Value&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Gender&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Male&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">32&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4623&#46;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>544&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;681&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Female&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">47&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4961&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>564&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " colspan="5" align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Severity &#40;spread of lesions&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Body area &#60;20&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">49&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4793&#46;9<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>486&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;071&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Body area 20&#37;&#8211;50&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">24&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4132&#46;7<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>435&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Body area &#62;50&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">7844&#46;7<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2928&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " colspan="5" align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Type&#58; localized or generalized&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Generalized&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">72&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4925&#46;9<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>434&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;421&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Localized&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3784&#46;3<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>541&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " colspan="5" align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Type&#58; segmental or non-segmental&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Segmental&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3668&#46;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>483&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;335&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Non-segmental&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">71&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4955&#46;0<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>439&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " colspan="5" align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Response to treatment&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">57&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4492&#46;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>380&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;154&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">21&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">5797&#46;1<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1083&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">78&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
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        "descripcion" => array:1 [
          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Comparison of serum concentrations of IL-27 based on demographic and clinical manifestations in vitiligo patients</p>"
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      "titulo" => "References"
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        0 => array:2 [
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            7 => array:3 [
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                  ]
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                0 => array:2 [
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                            0 => "J&#46; Wu"
                            1 => "M&#46; Zhou"
                            2 => "Y&#46; Wan"
                            3 => "A&#46; Xu"
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                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3892/mmr.2012.1117"
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              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
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                            1 => "P&#46; Rod Dunbar"
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                  ]
                  "host" => array:1 [
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                0 => array:2 [
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Journal Information
Vol. 95. Issue 5.
Pages 570-574 (1 September 2020)
Visits
6239
Vol. 95. Issue 5.
Pages 570-574 (1 September 2020)
Investigation
Open Access
Decreased levels of interleukin 27 in the serum of vitiligo patients
Visits
6239
Saeed Malek Hosseinia, Naser Gholijanib, Nooshafarin Chenaria, Kurosh Kalantara,
Corresponding author
kalantark@sums.ac.ir

Corresponding author.
a Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
b Autoimmune Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
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Table 1. Demographic analysis of population included in this study
Table 2. Comparison of serum concentrations of IL-27 based on demographic and clinical manifestations in vitiligo patients
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Abstract
Background

Vitiligo is a common skin disorder in which melanocytes are destroyed by auto-reactive immune responses. The loss of melanocytes results in the appearance of depigmented areas in different parts of the body. Cytokines have remarkable roles in the pathogenesis of vitiligo, such as IL-1, IL-6, and TNF-α; interleukin 27 (IL-27) is a new member of the IL-6/IL-12 family, mainly released by activated antigen-presenting cells. IL-27 has been suggested to function as a pro-inflammatory as well as an anti-inflammatory cytokine. Altered concentrations of IL-27 have been shown in various auto-immune diseases such as multiple sclerosis, rheumatoid arthritis, and psoriasis. No studies have been conducted to determine the expression of this cytokine in vitiligo patients.

Objective

The objective of this study was to determine the serum concentration of IL-27 in vitiligo patients and compare it with normal individuals.

Methods

The serum concentration of IL-27 in 79 vitiligo patients was evaluated in comparison to 45 healthy controls using ELISA assay.

Results

Results showed decreased concentration of IL-27 in vitiligo patients as compared with healthy subjects (p=0.026). Furthermore, no correlation between IL-27 concentrations and disease parameters such as vitiligo severity and the extension of the depigmented area was observed.

Study limitation

A larger sample size would be more recommended for this study.

Conclusion

The reduction in the serum levels of IL-27 in vitiligo patients compared to normal subjects suggested the possible anti-inflammatory role of this cytokine in vitiligo. Thus, IL-27 may be considered as a new target for the manipulation of the immune system in vitiligo patients.

Keywords:
Cytokines
Inflammation
Vitiligo
Full Text
Introduction

Vitiligo is a common acquired depigmentation disorder characterized by white patches which have been distributed in several parts of the body and greatly affects the quality of life and mental health of the patients.1,2 The prevalence of vitiligo in different societies was published in a range of 0.5% to 1%.3 According to a recent meta-analysis, the prevalence of vitiligo among different communities was 0.2%, and from hospital-based studies, it was 1.8%.4 Vitiligo emerges with a notable pattern of macular depigmentation, with a variable appearance in shape or size. This disease is classified into two subgroups, generalized and localized, in both of them insufficiency of melanocyte function can be observed.5

The main causes of vitiligo are complex and maybe stem from genetic disorders, which can feature polygenic and multifactorial inheritance.6 The main cause is not yet clear; however, some evidence has shown that exchange in immunological programs contributed to this kind of action.7 Vitiligo is assumed to result from autoimmune reactions that gradually destroy melanocytes. Despite the detection of self-reacting auto-antibodies in vitiligo patients, increasing evidence supports impaired cellular immunity as the main cause of this disfiguring disease.8 Cytokines have a pivotal role as mediators of cellular and humoral immune reactions. The imbalance between pro- and anti-inflammatory cytokines favoring the dominance of a Th1/Th17 response rather than a Th2/Treg response has been proposed as a possible underlying mechanism of vitiligo. However, there is considerable evidence in support of the role of CD8 type 1 T cells in the destruction of melanocytes.9,10 Increased expression of pro-inflammatory cytokines in vitiligo patients – including IL-1, IL-6, and TNF-α has been shown in several studies.11–13

IL-27, a rather new member of the IL-6/IL-12 family, is mainly released by activated antigen-presenting cells, including dendritic cells (DCs), monocytes, and macrophages.14,15 It is composed of two subunits, EBV-induced 3 (EBI3), an IL-12 p40 homolog, and p28, an IL-6 p35 homolog. The IL-27 receptor (IL-27R) is a heterodimer composed of an IL-27R specific alpha chain (WSX-1) and a gp130 subunit.16 The IL-27R is expressed by several cell types including but not limited to T, B, and NK cells, DCs, macrophages, keratinocytes, CD8 type1 T-cells, and endothelial cells.17–19 IL-27R signaling results in the activation of JAK-STAT and p38 MAPK pathways.20,21 IL-27 boosts the differentiation of Th1 and Tr1 cells through activation of STAT1/3 while inhibiting the differentiation of regulatory T-cells and Th2 cells.21–26 Besides Th1 differentiation, IL-27 signaling through STAT-1 has also been demonstrated to activate NK cells, which have been considered to have a role in the pathogenesis of vitiligo.27

Although previous studies identified the inflammatory role of IL-27, the latest evidence suggests an immunomodulatory effect of this cytokine.28 IL-27 induces Tr1 and inhibits Th2 and Th17 responses, therefore limiting the severity of autoimmune diseases by the suppression of Th17 cells.29–31 It has also immune-regulatory functions due to the up-regulation of PD-L1, IDO, and IL-10.32 Thus, it has suggested that this cytokine may be considered as a possible therapeutic agent for some inflammatory or autoimmune diseases.

Regarding the complex pro-inflammatory and anti-inflammatory nature of IL-27, several studies have investigated the local or systemic concentrations of IL-27 in different diseases. Alteration in the concentration of IL-27 and its correlation with autoimmune parameters have also been reported in several Th1/Th17-mediated inflammatory disorders, such as multiple sclerosis, systemic lupus erythematosus, inflammatory bowel disease, and rheumatoid arthritis.33–36

The concentrations of IL-27 in vitiligo patients have not yet been investigated. The present examination intended to evaluate the serum levels of IL-27 in vitiligo patients.

MethodsStudy population

Seventy-nine vitiligo patients including 32 (41%) males and 47 (59%) females, and 45 age and sex-matched healthy controls with no sign of autoimmune diseases were enrolled in the study. The study population included 72 patients with generalized vitiligo and seven cases with localized vitiligo. Participants in this study assented to join according to the ethics committee of the medical school of Shiraz University of Medical Sciences (IR.sums.med.rec.1397.400).

Sample collection

From all study populations, 5mL of peripheral blood was collected. After centrifugation at 3000rpm for 10min, the sera were separated and kept at −70°C until used.

Measurement of serum IL-27

Serum concentrations of IL-27 were distinguished by enzyme-linked immunosorbent assay (ELISA) in vitiligo patients and healthy subjects. IL-27 concentrations were quantified using an ELISA kit (DY2526-05, R&D Systems – United States) manufacturers’ instructions. The sensitivity of tests was 12.8pg/mL. Briefly, 100μL of capture antibody was coated in each well of a 96-well microplate for overnight at room temperature (RT). After washing and blocking, 100μL serum samples of patients and controls were added for 2h, at RT. Plates were washed again and 100μL of the detection antibody and then 100μL of the working dilution of streptavidin horseradish peroxidase was added for 20min. Then substrate solution (100μL) was added for 20min; finally, the reaction was stopped by adding stop solution to each well. The optical density of samples was read using a microplate reader at 450nm. The levels of cytokine were extrapolated from the related standard curve.

Statistical analysis

All data were analyzed by SPSS v. 16 software (SPSS Inc. – Chicago, IL, United States) and according to the normality test (Kolmogorov-Smirnov test); non-parametric (Mann–Whitney U and Kruskal–Wallis) tests were used; p-values<0.05 were considered as significant. Graphs were constructed using Graph Pad Prism (v. 6 – La Jolla, CA, United States). All data are presented as mean±standard error of the mean (SEM), unless otherwise specified.

ResultsDemographic analysis

An overview of the demographic and clinical features of vitiligo patients and healthy subjects participated in the study is provided in Table 1.

Table 1.

Demographic analysis of population included in this study

Study population  Patients  Controls 
Number  79  45 
Age  36.37±14.7  35.06±11.5 
Sex
Male  32 (41%)  18 (40%) 
Female  47 (59%)  27 (60%) 
Measurement of serum IL-27

The serum concentrations of IL-27 in 79 patients with generalized (72 cases) or localized (seven cases) vitiligo were observed using the ELISA technique. Forty-five age and sex-matched healthy subjects were used as the control group. As presented in Figure 1, the results showed that there was a significant difference in serum concentrations of IL-27 between vitiligo patients (5267.8±399pg/mL) and healthy subjects (7097.7±1502pg/mL) (p=0.0262).

Figure 1.

Mean serum concentration of IL-27 (pg/mL) in vitiligo patients and control group.

(0.06MB).
Correlations of IL-27 with demographic information and clinical manifestations

The correlations between serum concentrations of IL-27 with gender, the severity of the disease, two types of (localized/generalized) vitiligo, segmental/nonsegmental forms of the disease, and response to treatment in patients with vitiligo were examined. There was no significant correlation between IL-27 levels and these parameters in the present study (Table 2).

Table 2.

Comparison of serum concentrations of IL-27 based on demographic and clinical manifestations in vitiligo patients

    n  Mean (pg/mL)  p-Value 
Gender  Male  32  4623.8±544.2  0.681 
  Female  47  4961.5±564.5   
  Total  79     
Severity (spread of lesions)  Body area <20%  49  4793.9±486.6  0.071 
  Body area 20%–50%  24  4132.7±435.1   
  Body area >50%  7844.7±2928.6   
  Total  79     
Type: localized or generalized  Generalized  72  4925.9±434.3  0.421 
  Localized  3784.3±541.5   
  Total  79     
Type: segmental or non-segmental  Segmental  3668.8±483.0  0.335 
  Non-segmental  71  4955.0±439.5   
  Total  79     
Response to treatment  Yes  57  4492.8±380.9  0.154 
  No  21  5797.1±1083.9   
  Total  78     
Discussion

The main reason for vitiligo is ill defined. Human and experimental studies have recently provided considerable evidence regarding the pattern of autoimmunity in the pathogenesis of vitiligo. Both humoral and cellular immunity have shown to be involved in the etiology of the disease.37–39 The accumulated evidence suggests a primary role for cell-mediated mechanisms, including Th1/Th17 and Tc1 cells in the pathogenesis of vitiligo.40 Various inflammatory cytokines (IL-1, IL-6, TNF-α, IL-6, and IL-17) have a key role in skin depigmentation, while the level of TGF-β indicated reversible state.6,41

IL-27 has been suggested to function as a pro-inflammatory as well as an anti-inflammatory cytokine. Altered concentrations of IL-27 have been shown in several autoimmune and skin disorders. Despite early studies identified pro-inflammatory role of IL-27, latest evidence suggests that it suppresses a range of immune cell proliferation and cytokine production.28 These cytokine applications are related to its pro- or anti-inflammatory activity. The dual role of IL-27 is related to the different tissues involved, the underlying mechanism, or the kind and stage of autoimmune diseases.29 Previous studies revealed that the blood concentration of IL-27 increased in pemphigus and psoriatic patients. They showed that IL-27 levels strongly correlated with the IgG auto-antibody titers in pemphigus, and also disease onset and severity of psoriasis.42,43 The expression of IL-27 in lesional eczematous skin has been reported too.43 This evidence supports a pro-inflammatory function of IL-27. In spite of skin disorders in which IL-27 plasma concentrations have increased, the serum level of this cytokine is reduced in some auto-immune diseases such as Vogt-Koyanagi-Harada syndrome (VKH), Behçet's disease (BD), and SLE. Wang and his colleagues described that the expression of IL-27 p28 mRNA by peripheral blood mononuclear cells (PBMCs) and serum concentration of IL-27 in the sera and supernatants of cultured PBMCs were noticeably reduced in patients with active BD and VKH. In addition, Gaber et al. have observed that the IL-27 level in SLE patients is markedly lower than in healthy controls.44–46

Based on those explanations and the results of several studies about the altered expression of IL-27 in autoimmunity and skin disorders, it was hypothesized that the concentration of IL-27 in vitiligo patients might be altered. Consistent with previous studies in SLE and BD patients, the present study showed that the concentrations of IL-27 serum levels were reduced in vitiligo patients, which can be justified through some probable mechanisms. First, IL-27 acts against Th17 development in a direct manner through modulating DCs and induces IL-10 production by naïve CD4+ T-cells.45 Th17 contributes to the pathogenesis of vitiligo by IL-17 production. IL-27 inhibits the differentiation and generation of Th17 cells via IL-6 and transforming growth factor-β (TGF-β) suppression, which is dependent on the intracellular signaling molecule STAT1.44

Conclusion

It can be concluded that IL-27 has an immunomodulatory role in vitiligo. According to the observations of the present study, there was no association between IL-27 serum level and demographic information, severity of the disease, types of vitiligo, and response to treatment in patients with vitiligo. Further studies with more patients of all types of vitiligo are needed to reveal the possible alterations in the concentration of IL-27 in those patients and their correlation with disease characteristics, including severity and the extension of the affected skin area. In conclusion, IL-27 has two roles; it should be considered as a new target for the manipulation of the immune system in various immune-mediated disorders.

Financial support

This study was supported by Shiraz University of Medical Sciences (grant No. 17684).

Authors’ contributions

Saeed Malek Hosseini: Drafting and editing of the manuscript.

Naser Gholijani: Collection, analysis, and interpretation of data; participation in the study design.

Nooshafarin Chenari: Collection, analysis, and interpretation of data; participation in the study design.

Nooshafarin Chenari: Drafting and editing of the manuscript.

Conflicts of interest

None declared.

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How to cite this article: Hosseini SM, Gholijani N, Chenari N, Kalantar K. Decreased levels of interleukin 27 in the serum of vitiligo patients. An Bras Dermatol. 2020;95:570–4.

Study conducted at the Shiraz University of Medical Sciences, Shiraz, Iran.

Copyright © 2020. Sociedade Brasileira de Dermatologia
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