Letter - Dermatopathology
A new technique of paraffin-embedding of formalin-fixed nail sample, obtained by tangential excision - potato as guide mold
Lido
3971
Vezesque se leu este artigo
1228
Total PDF
2743
Total HTML
Compartilhar estatÃsticas
array:23 [ "pii" => "S0365059623001897" "issn" => "03650596" "doi" => "10.1016/j.abd.2023.01.005" "estado" => "S300" "fechaPublicacion" => "2024-01-01" "aid" => "824" "copyright" => "Sociedade Brasileira de Dermatologia" "copyrightAnyo" => "2023" "documento" => "article" "crossmark" => 1 "subdocumento" => "crp" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "Traduccion" => array:1 [ "pt" => array:18 [ "pii" => "S2666275223002199" "issn" => "26662752" "doi" => "10.1016/j.abdp.2023.09.001" "estado" => "S300" "fechaPublicacion" => "2024-01-01" "aid" => "824" "copyright" => "Sociedade Brasileira de Dermatologia" "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "pt" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Carta – Dermatopatologia</span>" "titulo" => "Nova técnica de inclusão em parafina de amostra de unha fixada em formalina, obtida por excisão tangencial – Molde de batata como guia" "tienePdf" => "pt" "tieneTextoCompleto" => "pt" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "139" "paginaFinal" => "141" ] ] "contieneTextoCompleto" => array:1 [ "pt" => true ] "contienePdf" => array:1 [ "pt" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figura 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 464 "Ancho" => 1755 "Tamanyo" => 219638 ] ] "descripcion" => array:1 [ "pt" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">(A) Melanoníquia – dermatoscopia de contato. (B‐C) Onicomatricoma pigmentado: amostra adequadamente incluída em corte longitudinal. Hematoxilina & eosina, ampliação original 40<span class="elsevierStyleHsp" style=""></span>×, 200<span class="elsevierStyleHsp" style=""></span>×.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Laura Bertanha, Cristina Diniz Borges Figueira de Mello, Ingrid Iara Damas, Rafael Fantelli Stelini, Nilton Di Chiacchio, Maria Letícia Cintra" "autores" => array:6 [ 0 => array:2 [ "nombre" => "Laura" "apellidos" => "Bertanha" ] 1 => array:2 [ "nombre" => "Cristina Diniz Borges Figueira de" "apellidos" => "Mello" ] 2 => array:2 [ "nombre" => "Ingrid Iara" "apellidos" => "Damas" ] 3 => array:2 [ "nombre" => "Rafael Fantelli" "apellidos" => "Stelini" ] 4 => array:2 [ "nombre" => "Nilton" "apellidos" => "Di Chiacchio" ] 5 => array:2 [ "nombre" => "Maria Letícia" "apellidos" => "Cintra" ] ] ] ] ] "idiomaDefecto" => "pt" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S0365059623001897" "doi" => "10.1016/j.abd.2023.01.005" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0365059623001897?idApp=UINPBA00008Z" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2666275223002199?idApp=UINPBA00008Z" "url" => "/26662752/0000009900000001/v1_202401040746/S2666275223002199/v1_202401040746/pt/main.assets" ] ] "itemSiguiente" => array:18 [ "pii" => "S0365059623002088" "issn" => "03650596" "doi" => "10.1016/j.abd.2023.02.008" "estado" => "S300" "fechaPublicacion" => "2024-01-01" "aid" => "840" "copyright" => "Sociedade Brasileira de Dermatologia" "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Letter - Tropical/Infectious and parasitic dermatology</span>" "titulo" => "Chromoblastomycosis in a renal transplant patient" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "142" "paginaFinal" => "145" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1288 "Ancho" => 2175 "Tamanyo" => 299596 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0015" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">(A) Velvety greenish-black culture, with a central elevation. Mycosel agar. (B) Microculture showing the presence of <span class="elsevierStyleItalic">Cladosporium</span>-type sporulation with erect conidiophores, and short chains with two to three conidia. (Staining: cotton blue). <span class="elsevierStyleItalic">Fonsecaea</span> spp</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Ingrid Rocha Meireles Holanda, Priscila Neri Lacerda, Carolina Nunhez da Silva, Rosangela Maria Pires de Camargo, Anna Carolina Miola, Silvio Alencar Marques" "autores" => array:6 [ 0 => array:2 [ "nombre" => "Ingrid Rocha Meireles" "apellidos" => "Holanda" ] 1 => array:2 [ "nombre" => "Priscila Neri" "apellidos" => "Lacerda" ] 2 => array:2 [ "nombre" => "Carolina Nunhez da" "apellidos" => "Silva" ] 3 => array:2 [ "nombre" => "Rosangela Maria Pires de" "apellidos" => "Camargo" ] 4 => array:2 [ "nombre" => "Anna Carolina" "apellidos" => "Miola" ] 5 => array:2 [ "nombre" => "Silvio Alencar" "apellidos" => "Marques" ] ] ] ] ] "idiomaDefecto" => "en" "Traduccion" => array:1 [ "pt" => array:9 [ "pii" => "S2666275223002370" "doi" => "10.1016/j.abdp.2023.09.018" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "pt" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2666275223002370?idApp=UINPBA00008Z" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0365059623002088?idApp=UINPBA00008Z" "url" => "/03650596/0000009900000001/v1_202401040817/S0365059623002088/v1_202401040817/en/main.assets" ] "itemAnterior" => array:18 [ "pii" => "S0365059623001940" "issn" => "03650596" "doi" => "10.1016/j.abd.2023.02.006" "estado" => "S300" "fechaPublicacion" => "2024-01-01" "aid" => "829" "copyright" => "Sociedade Brasileira de Dermatologia" "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Letter - Dermatopathology</span>" "titulo" => "A unique case of a lymphoproliferative disorder affecting the skin and uterine cervix on a male transgender" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "135" "paginaFinal" => "139" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 2777 "Ancho" => 3341 "Tamanyo" => 1384224 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0010" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">(A‒H) Skin histopathology: (Hematoxylin & eosin, ×10 and ×200), there is a “bottom-heavy” infiltrate of small lymphocytes, with a nodular growth pattern and no epidermal alterations; CD20 (×10) highlighting neoplastic B-cells; CD3 (×10) showing the associated rich T-cell infiltrate; CD21 showing areas with follicle outlines; Bcl2 positive (×10) in B and T cells; Bcl6 positive (×10 and ×40) inside and outside the germinal centers</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Jade Cury-Martins, Marcelo A. Giannotti, Denis Miyashiro, Juliana Pereira, José Antonio Sanches" "autores" => array:5 [ 0 => array:2 [ "nombre" => "Jade" "apellidos" => "Cury-Martins" ] 1 => array:2 [ "nombre" => "Marcelo A." "apellidos" => "Giannotti" ] 2 => array:2 [ "nombre" => "Denis" "apellidos" => "Miyashiro" ] 3 => array:2 [ "nombre" => "Juliana" "apellidos" => "Pereira" ] 4 => array:2 [ "nombre" => "José Antonio" "apellidos" => "Sanches" ] ] ] ] ] "idiomaDefecto" => "en" "Traduccion" => array:1 [ "pt" => array:9 [ "pii" => "S2666275223002254" "doi" => "10.1016/j.abdp.2023.09.007" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "pt" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2666275223002254?idApp=UINPBA00008Z" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0365059623001940?idApp=UINPBA00008Z" "url" => "/03650596/0000009900000001/v1_202401040817/S0365059623001940/v1_202401040817/en/main.assets" ] "en" => array:17 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Letter - Dermatopathology</span>" "titulo" => "A new technique of paraffin-embedding of formalin-fixed nail sample, obtained by tangential excision - potato as guide mold" "tieneTextoCompleto" => true "saludo" => "Dear Editor," "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "139" "paginaFinal" => "141" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Laura Bertanha, Cristina Diniz Borges Figueira de Mello, Ingrid Iara Damas, Rafael Fantelli Stelini, Nilton Di Chiacchio, Maria Letícia Cintra" "autores" => array:6 [ 0 => array:4 [ "nombre" => "Laura" "apellidos" => "Bertanha" "email" => array:1 [ 0 => "labertanha@yahoo.com" ] "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:3 [ "nombre" => "Cristina Diniz Borges Figueira" "apellidos" => "de Mello" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 2 => array:3 [ "nombre" => "Ingrid Iara" "apellidos" => "Damas" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 3 => array:3 [ "nombre" => "Rafael Fantelli" "apellidos" => "Stelini" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 4 => array:3 [ "nombre" => "Nilton" "apellidos" => "Di Chiacchio" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 5 => array:3 [ "nombre" => "Maria Letícia" "apellidos" => "Cintra" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brazil" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Hospital do Servidor Público Municipal, São Paulo, SP, Brazil" "etiqueta" => "b" "identificador" => "aff0010" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1000 "Ancho" => 2925 "Tamanyo" => 359125 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0355" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">(A) The nail matrix biopsy sample is laid flat on a piece of filter paper, in the place corresponding to the drawn diagram. (B) A potato piece of 2<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>1.5<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>0.5<span class="elsevierStyleHsp" style=""></span>cm with a slit made in it by means of a scalpel. (C) The piece of potato, with the tissue inside. (D) Longitudinal section demonstrating the components of the nail apparatus. Hematoxylin & eosin, original magnification ×40.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">The specimen obtained by tangential excision of the nail bed/matrix is thin and fragile and often bends after being immersed in a fixative medium. This makes it difficult to guide the orientation and paraffin embedding of the sample. The epidermal surface is normally easily identifiable on skin biopsies due to a shiny dermis. However, biopsies of the nail matrix or nail bed usually lack a nail plate, which, if present, could help identify the top of the specimen. Without the nail, both tissue surfaces are shiny.<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> Consequently, due to an embedding defect, the pathologist has trouble identifying the different regions of the nail unit and analyzing the morphological changes.<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Techniques for affixing the material to the paper on which the nail apparatus was drawn have been described. They aim to guide the epithelial portion and the site from which the biopsy was obtained.<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2–4</span></a> However, even if all these techniques produce a clear distinction of the epithelial portion and adequate signaling of the biopsy site, loss of orientation may occur at the time of tissue paraffin embedding. If the orientation is compromised, for example, the normal epidermis can simulate a papilloma, resulting in misdiagnosis.</p><p id="par0015" class="elsevierStylePara elsevierViewall">We aimed to evaluate the effectiveness of specimen embedding methodology, using a potato (<span class="elsevierStyleItalic">Solanum tuberosum</span>) as a guide mold, after approval of the ethics committee (CAAE: 48711821.0.3001.5442).</p><p id="par0020" class="elsevierStylePara elsevierViewall">Two samples obtained for the investigation of nail melanonychia were selected. They were laid flat on a piece of filter paper, in the place corresponding to the drawn diagram of the nail apparatus, with the matrix epithelium facing upwards. The paper was folded to wrap the material, then stapled, and the envelope was immersed in 10% formalin.<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> At the laboratory, the samples were removed from the filter paper, and inserted into a groove produced in a slice of potato (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>). This had previously been prepared by cutting into pieces measuring approximately 2<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>1.5<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>0.5<span class="elsevierStyleHsp" style=""></span>cm and kept immersed in 10% formalin for at least 24<span class="elsevierStyleHsp" style=""></span>hours, and up to 30 days, to avoid its softening. The piece of potato, with the tissue inside, was placed in a cassette for histological processing. Implementing this method resulted in perfect inclusion, with good longitudinal exposure of the nail components involved by the tuber cells (<a class="elsevierStyleCrossRefs" href="#fig0005">Figs. 1 and 2</a>).</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">Proper microscopic analysis of thin nail bed samples depends on suitable surgical technique, preparation, tissue fixation, and histotechnical processing, especially avoiding embedding artifacts. Inadequate surgical dissection and/or sampling or iatrogenically induced artifacts might invalidate the microscopic interpretation.<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">In our experience, the use of potatoes as support minimizes embedding artifacts and can be a useful tool in the arsenal of care for thin and delicate tissue samples, such as those obtained from nail tangential surgeries.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Financial support</span><p id="par0035" class="elsevierStylePara elsevierViewall">None declare.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Authors' contributions</span><p id="par0040" class="elsevierStylePara elsevierViewall">Laura Bertanha: Participated in generating and analyzing the data; wrote the majority of the original draft of the paper, reviewed the pertinent raw data on which the results and conclusions of this study are based, and approved the final version of this paper.</p><p id="par0045" class="elsevierStylePara elsevierViewall">Cristina Diniz Borges Figueira de Mello: Participated in writing the paper, reviewed the pertinent raw data on which the results and conclusions of this study are based, and approved the final version of this paper.</p><p id="par0050" class="elsevierStylePara elsevierViewall">Ingrid Iara Damas: Participated in generating data, writing the paper and approving the final version of this paper.</p><p id="par0055" class="elsevierStylePara elsevierViewall">Rafael Fantelli Stelini: Participated in generating data, writing the paper, reviewing the pertinent raw data on which the results and conclusions of this study are based and approving the final version of this paper.</p><p id="par0060" class="elsevierStylePara elsevierViewall">Nilton Di Chiacchio: Participated in generating data, writing the paper, reviewing the pertinent raw data on which the results and conclusions of this study are based and approved the final version of this paper.</p><p id="par0065" class="elsevierStylePara elsevierViewall">Maria Letícia Cintra: Participated in designing, generating, and analyzing the data; Wrote the paper and reviewed the pertinent raw data on which the results and conclusions of this study are based and approved the final version of this paper</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Conflicts of interest</span><p id="par0070" class="elsevierStylePara elsevierViewall">None declared.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Financial support" ] 1 => array:2 [ "identificador" => "sec0010" "titulo" => "Authors' contributions" ] 2 => array:2 [ "identificador" => "sec0015" "titulo" => "Conflicts of interest" ] 3 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2022-11-29" "fechaAceptado" => "2023-01-01" "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Study conducted at the Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brazil and Municipal Public Servant's Hospital, São Paulo, SP, Brazil.</p>" ] ] "multimedia" => array:2 [ 0 => array:8 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1000 "Ancho" => 2925 "Tamanyo" => 359125 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0355" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">(A) The nail matrix biopsy sample is laid flat on a piece of filter paper, in the place corresponding to the drawn diagram. (B) A potato piece of 2<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>1.5<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>0.5<span class="elsevierStyleHsp" style=""></span>cm with a slit made in it by means of a scalpel. (C) The piece of potato, with the tissue inside. (D) Longitudinal section demonstrating the components of the nail apparatus. Hematoxylin & eosin, original magnification ×40.</p>" ] ] 1 => array:8 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 464 "Ancho" => 1755 "Tamanyo" => 218388 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at0360" "detalle" => "Figure " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">(A) Melanonychia – contact dermoscopy. (B‒C) Pigmented onychomatricoma: The specimen adequately embedded in longitudinal section. Hematoxylin & eosin, original magnification ×40, ×200.</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:5 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Marking of nail matrix biopsies with ink aids in proper specimen orientation for more accurate histologic evaluation" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "R. George" 1 => "S. Clarke" 2 => "M. Ioffreda" 3 => "E. Billingsley" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1524-4725.2008.34355.x" "Revista" => array:6 [ "tituloSerie" => "Dermatol Surg." "fecha" => "2008" "volumen" => "34" "paginaInicial" => "1705" "paginaFinal" => "1706" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19076896" "web" => "Medline" ] ] ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0010" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "How to submit a nail specimen" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "E. Reinig" 1 => "P. Rich" 2 => "C.T. Thompson" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.det.2014.12.012" "Revista" => array:6 [ "tituloSerie" => "Dermatol Clin." "fecha" => "2015" "volumen" => "33" "paginaInicial" => "303" "paginaFinal" => "307" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25828721" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0015" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Submitting tangential nail-matrix specimens" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "J. Ocampo-Garza" 1 => "N.G. Di Chiacchio" 2 => "J. Dominguez-Cherit" 3 => "L. Fonseca Noriega" 4 => "N. Di Chiacchio" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "J Am Acad Dermatol." "fecha" => "2017" "volumen" => "77" "paginaInicial" => "133" "paginaFinal" => "134" ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0020" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Tangential excision of pigmented nail matrix lesions responsible for longitudinal melanonychia: evaluation of the technique on a series of 30 patients" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "B. Richert" 1 => "A. Theunis" 2 => "S. Norrenberg" 3 => "J. André" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Am Acad Dermatol" "fecha" => "2013" "volumen" => "69" "paginaInicial" => "96" "paginaFinal" => "104" "itemHostRev" => array:3 [ "pii" => "S0735109714059415" "estado" => "S300" "issn" => "07351097" ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0025" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Clinical pathologic correlations for diagnosis and treatment of nail disorders" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "O.I. Kovich" 1 => "A.C. Soldano" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1529-8019.2007.00107.x" "Revista" => array:7 [ "tituloSerie" => "Dermatol Ther." "fecha" => "2007" "volumen" => "20" "paginaInicial" => "11" "paginaFinal" => "16" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17403256" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S2254887421001636" "estado" => "S300" "issn" => "22548874" ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/03650596/0000009900000001/v1_202401040817/S0365059623001897/v1_202401040817/en/main.assets" "Apartado" => array:4 [ "identificador" => "95152" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Letter - Dermatopathology" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/03650596/0000009900000001/v1_202401040817/S0365059623001897/v1_202401040817/en/main.pdf?idApp=UINPBA00008Z&text.app=https://clinics.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0365059623001897?idApp=UINPBA00008Z" ]
| Ano/Mês | Html | Total | |
|---|---|---|---|
| 2024 Novembro | 17 | 7 | 24 |
| 2024 Outubro | 122 | 75 | 197 |
| 2024 Setembro | 188 | 89 | 277 |
| 2024 Agosto | 185 | 113 | 298 |
| 2024 Julho | 181 | 120 | 301 |
| 2024 Junho | 174 | 64 | 238 |
| 2024 Maio | 138 | 64 | 202 |
| 2024 Abril | 222 | 102 | 324 |
| 2024 Março | 201 | 68 | 269 |
| 2024 Fevereiro | 514 | 106 | 620 |
| 2024 Janeiro | 421 | 104 | 525 |
| 2023 Dezembro | 97 | 66 | 163 |
| 2023 Novembro | 127 | 75 | 202 |
| 2023 Outubro | 89 | 83 | 172 |
| 2023 Setembro | 62 | 87 | 149 |
| 2023 Agosto | 5 | 5 | 10 |
Mostrar tudo
