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Histopathological aspects of the inclusion of surgical material in micrographic surgery using the Munich method and its comparison with horizontal histological sections
Airá Novello Vilar
, Arthur César Farah Ferreira
Corresponding author
Private Clinic, Concórdia, SC, Brazil
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"airanovellovilar@hotmail.com" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "*" "identificador" => "cor0005" ] ] ] 1 => array:2 [ "nombre" => "Arthur César Farah" "apellidos" => "Ferreira" ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Private Clinic, Concórdia, SC, Brazil" "identificador" => "aff0005" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">The Munich method for micrographic surgery is technically distinct from the Mohs technique, both regarding the surgery itself and laboratory processing, as well as the microscopic analysis. The surgical specimen is usually examined without division, as long as its size allows for full inclusion.<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">1</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">In the Munich technique, originally described in 1992 and published in Germany in 1995, the surgical specimen is frozen, usually outside the cryostat, by a direct stream of CO<span class="elsevierStyleInf">2</span> and with the use of distilled water, and then inserted in the cryostat to be sliced.<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">2</span></a> However, we have, similarly to other colleagues, frozen the specimen directly in the cryostat with the use of OCT, as is customary in the intraoperative technique not only for skin, but for several other tissues.<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">3,4</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">Presented as a “new way of assessing debulking,” from the technical and laboratory standpoint, the method described by Portela et al.<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">5</span></a> with horizontal sections, is identical to the Munich technique, despite starting from the surface to the depth and the fact that the interval and the thickness of sections are different, which may vary due to the peculiarities of each tissue. Likewise, the observation of the tumor and its relationship with the surgical margins is one of the most striking features of the Munich method.</p><p id="par0020" class="elsevierStylePara elsevierViewall">Moreover, the aforementioned authors confuse the surgical margin with the surgical border, stating that the Mohs method, which is peripheral, examines the surgical margin and not the hypothetical surgical border (<span class="elsevierStyleItalic">i.e</span>., the section that is deposited on the microscope slide after the sectioning of the block).</p><p id="par0025" class="elsevierStylePara elsevierViewall">While perhaps not identical, the Munich technique should at least have been referred to by the authors as the original idea, since it has been widely described in the literature, including in Anais Brasileiros de Dermatologia.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Financial support</span><p id="par0030" class="elsevierStylePara elsevierViewall">None declared.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Authors' contributions</span><p id="par0035" class="elsevierStylePara elsevierViewall">Airá Novello Vilar: Approval of the final version of the manuscript; conception and planning of the study; drafting and editing of the manuscript.</p><p id="par0040" class="elsevierStylePara elsevierViewall">Arthur César Farah Ferreira: Critical review of the literature; critical review of the manuscript.</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Conflicts of interest</span><p id="par0045" class="elsevierStylePara elsevierViewall">None declared.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Financial support" ] 1 => array:2 [ "identificador" => "sec0010" "titulo" => "Authors' contributions" ] 2 => array:2 [ "identificador" => "sec0015" "titulo" => "Conflicts of interest" ] 3 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2020-02-27" "fechaAceptado" => "2020-04-10" "NotaPie" => array:2 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">How to cite this article: Vilar AN, Ferreira ACF. Histopathological aspects of the inclusion of surgical material in micrographic surgery using the Munich method and its comparison with horizontal histological sections. An Bras Dermatol. 2020;95:547–8.</p>" ] 1 => array:2 [ "etiqueta" => "☆☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0010">Study conducted at the Private Clinic, Concórdia, SC, Brazil.</p>" ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0015" "bibliografiaReferencia" => array:5 [ 0 => array:3 [ "identificador" => "bib0030" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The fundamental differences among the variations of micrografic surgery" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "L.F.F. Kopke" 1 => "B. 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Lo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1524-4725.1991.tb03274.x" "Revista" => array:6 [ "tituloSerie" => "J Dermatol Surg Oncol" "fecha" => "1991" "volumen" => "17" "paginaInicial" => "857" "paginaFinal" => "860" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1757646" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0050" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Horizontal histological sections in the preliminary evaluation of basal cell carcinoma submitted to Mohs micrographic surgery" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "P.S. Portela" 1 => "D.A. Teixeira" 2 => "C.D.A.S. Machado" 3 => "M.A.S. Pinhal" 4 => "F.M. Paschoal" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.abd.2017.11.001" "Revista" => array:6 [ "tituloSerie" => "An Bras Dermatol" "fecha" => "2019" "volumen" => "94" "paginaInicial" => "671" "paginaFinal" => "676" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31789269" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/03650596/0000009500000004/v3_202008041755/S0365059620301422/v3_202008041755/en/main.assets" "Apartado" => array:4 [ "identificador" => "81766" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Correspondence" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/03650596/0000009500000004/v3_202008041755/S0365059620301422/v3_202008041755/en/main.pdf?idApp=UINPBA00008Z&text.app=https://clinics.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0365059620301422?idApp=UINPBA00008Z" ]
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